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KMID : 0617220000110010115
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Duksung Bulletin Phamaceutical Sciences
2000 Volume.11 No. 1 p.115 ~ p.124
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Induction of CTL responses and identification of a novel epitope of hepatitis B virus surface antigens in C57BL/6 mice immunized with recombinant vaccinia viruses
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Roh, Sujin
Lee, Yun-Kyung/Ahn, Byung-Yoon/Kim, Kilhyoun/Moon, Aree
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Abstract
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MHC class I-restricted cytotoxic T lymphocyte (CTL) response to hepatitis B virus (HBV) surface antigens (HBsAg) has been suggested to play essential roles in viral clearance and pathogenesis of HBV-induced hepatitis. In the present study, we analyzed CTL responses to endogenously synthesized or exogenously introduced HBsAg in C57BL/6 mice (H-2^b). We show that the endogenously synthesized surface antigens of adr-type HBV encoded by recombinant vaccinia virus efficiently elicit CTL responses in C57BL/6 mice previously defined as non-responders to vaccinia-HBV immunization. We also show that two peptides. S_179-186(FVQWFVGL) and S_208-216 (ILSPFLPLL), serve as effective motifs for CTL response in H-2^b system after in vitro restimulation of the primed T cells with either of the two synthetic peptides. S_208-215 has recently been identified as a CTL epitope which could be produced by exogenous pathway only, in contrast to the current result, while S_179-186 appeared a novel epitope for CTL response. In addition, we show that soluble HBsAg also elicits CTL responses in H-2^b mice upon in vitro restimulation with the two peptides, although less efficiently compared with the recombinant vaccinia viruses. These findings may provide an efficient experimental system for studying H-2^b-restricted immune responses against endogenously synthesized and exogenously introduced HBsAg. ⓒ 2001 Elsevier Science B.V. All rights reserved.
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